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5–4 μmol/L vary,[156][157] the concentration of serotonin in synaptic vesicles was measured at 270 mM.[182] Following vesicular release, the resulting concentration of serotonin within the synaptic cleft, to which serotonin receptors are uncovered, is believed to be about 300 μmol/L. As a result, although in vitro receptor binding affinities,
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